@article {13632,
	title = {Evolution of nuclear- and mitochondrial-encoded subunit interaction in cytochrome c oxidase},
	journal = {Mol Biol Evol},
	volume = {18},
	number = {4},
	year = {2001},
	note = {<p>Mol Biol Evol. 2001 Apr;18(4):563-9.</p>},
	month = {Apr},
	pages = {563-9},
	type = {Research Support, Non-U.S. Gov{\textquoteright}tResearch Support, U.S. Gov{\textquoteright}t, Non-P.H.S.Research Support, U.S. Gov{\textquoteright}t, P.H.S.},
	edition = {2001/03/27},
	abstract = {<p>Mitochondrial DNA (mtDNA)-encoded proteins function in eukaryotes as subunits of respiratory complexes that also contain nuclear DNA (nDNA)-encoded subunits. The importance of functional interactions between mtDNA- and nDNA-encoded proteins was previously demonstrated by testing the survivability of cybrid cells or individuals containing nDNA and mtDNA from different populations or species. This report focuses on the multisubunit respiratory complex cytochrome c oxidase (COX), made up of both mtDNA-encoded and nDNA-encoded subunits. A combination of evolutionary and crystallographic data is employed to determine whether rates of nonsynonymous substitutions have been higher, the same, or lower for residues in close proximity that are encoded by a different genome (nDNA or mtDNA). This determination is performed by simply taking the ratio, called the interaction ratio i, of the nonsynonymous substitution rate of the close-contact residues to the nonsynonymous substitution rate of the noncontact residues. We assume that the close-contact residues (which are more likely to interact) are functionally important and that, therefore, amino acid replacements among these residues cannot escape the scrutiny of natural selection. i = 1 indicates that the close-contact residues have been under neither greater purifying selection nor greater positive selection than the noncontact residues as a specific consequence of their being encoded by separate genomes. i \&lt; 1 indicates that the close-contact residues have been under greater purifying selection but less positive selection than have the noncontact residues. Conversely, i \&gt; 1 indicates that the close-contact residues have been under less purifying but greater positive selection than have the noncontact residues. i \&lt; 1 may be referred to as a constraining interaction; i.e., the close-contact residues compared with the noncontact residues appear to be under greater structural-functional constraints. On the other hand, i \&gt; 1 may be referred to as an optimizing interaction; i.e., apparently many different amino acid replacements are required to optimize this subunit{\textquoteright}s interaction with the other subunit. A major finding is that the nDNA-encoded residues in close physical proximity to mtDNA-encoded residues evolve more slowly than the other nuclear-encoded residues (and thus display a constraining interaction), whereas the mtDNA-encoded residues in close physical proximity to nDNA-encoded residues evolve more rapidly than the other mitochondrial-encoded residues (and thus display an optimizing interaction). A possible reason for this striking difference between the nuclear- and mitochondrial-encoded COX subunits in how their functional interaction evolves is discussed.</p>},
	keywords = {*Evolution, Amino Acid Substitution/*genetics, Animals, DNA, DNA/analysis/genetics, Electron Transport Complex IV/*genetics/metabolism, Humans, Macromolecular Substances, Mitochondrial/genetics, Models, Molecular, Phylogeny, Protein Subunits},
	isbn = {0737-4038 (Print)0737-40},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/11264408},
	author = {Schmidt, T. R. and Wu, W. and Goodman, M. and Grossman, L. I.}
}
