@article {20321, title = {Metabolism of vertebrate amino sugars with N-glycolyl groups: mechanisms underlying gastrointestinal incorporation of the non-human sialic acid xeno-autoantigen N-glycolylneuraminic acid}, journal = {J Biol Chem}, volume = {287}, number = {34}, year = {2012}, note = {

J Biol Chem.\ 2012 Aug 17;287(34):28852-64. doi: 10.1074/jbc.M112.364182. Epub 2012 Jun 12.

}, month = {Aug 17}, pages = {28852-64}, type = {Research Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov{\textquoteright}t}, edition = {2012/06/14}, abstract = {

Although N-acetyl groups are common in nature, N-glycolyl groups are rare. Mammals express two major sialic acids, N-acetylneuraminic acid and N-glycolylneuraminic acid (Neu5Gc). Although humans cannot produce Neu5Gc, it is detected in the epithelial lining of hollow organs, endothelial lining of the vasculature, fetal tissues, and carcinomas. This unexpected expression is hypothesized to result via metabolic incorporation of Neu5Gc from mammalian foods. This accumulation has relevance for diseases associated with such nutrients, via interaction with Neu5Gc-specific antibodies. Little is known about how ingested sialic acids in general and Neu5Gc in particular are metabolized in the gastrointestinal tract. We studied the gastrointestinal and systemic fate of Neu5Gc-containing glycoproteins (Neu5Gc-glycoproteins) or free Neu5Gc in the Neu5Gc-free Cmah(-/-) mouse model. Ingested free Neu5Gc showed rapid absorption into the circulation and urinary excretion. In contrast, ingestion of Neu5Gc-glycoproteins led to Neu5Gc incorporation into the small intestinal wall, appearance in circulation at a steady-state level for several hours, and metabolic incorporation into multiple peripheral tissue glycoproteins and glycolipids, thus conclusively proving that Neu5Gc can be metabolically incorporated from food. Feeding Neu5Gc-glycoproteins but not free Neu5Gc mimics the human condition, causing tissue incorporation into human-like sites in Cmah(-/-) fetal and adult tissues, as well as developing tumors. Thus, glycoproteins containing glycosidically linked Neu5Gc are the likely dietary source for human tissue accumulation, and not the free monosaccharide. This human-like model can be used to elucidate specific mechanisms of Neu5Gc delivery from the gut to tissues, as well as general mechanisms of metabolism of ingested sialic acids.

}, keywords = {*Meat Products, Animals, Antigens, Autoantigens/*metabolism, Gastrointestinal Tract/*metabolism, Glycoproteins/genetics/*metabolism, Heterophile/*metabolism, Humans, Knockout, Mice, Neuraminic Acids/*metabolism, Species Specificity}, isbn = {1083-351X (Electronic)00}, url = {http://www.ncbi.nlm.nih.gov/pubmed/22692204}, author = {Banda, K. and Gregg, C. J. and Chow, R. and Nissi M Varki and Ajit Varki} }