@article {308689,
	title = {A highly abundant bacteriophage discovered in the unknown sequences of human faecal metagenomes.},
	journal = {Nat Commun},
	volume = {5},
	year = {2014},
	note = {http://www.nature.com/ncomms/2014/140724/ncomms5498/full/ncomms5498.html},
	month = {2014},
	pages = {4498},
	abstract = {<p>Metagenomics, or sequencing of the genetic material from a complete microbial community, is a promising tool to discover novel microbes and viruses. Viral metagenomes typically contain many unknown sequences. Here we describe the discovery of a previously unidentified bacteriophage present in the majority of published human faecal metagenomes, which we refer to as crAssphage. Its ~97 kbp genome is six times more abundant in publicly available metagenomes than all other known phages together; it comprises up to 90\% and 22\% of all reads in virus-like particle (VLP)-derived metagenomes and total community metagenomes, respectively; and it totals 1.68\% of all human faecal metagenomic sequencing reads in the public databases. The majority of crAssphage-encoded proteins match no known sequences in the database, which is why it was not detected before. Using a new co-occurrence profiling approach, we predict a Bacteroides host for this phage, consistent with Bacteroides-related protein homologues and a unique carbohydrate-binding domain encoded in the phage genome.</p>},
	keywords = {Bacteriophages, Bacteroides, Clustered Regularly Interspaced Short Palindromic Repeats, Feces, Female, Humans, Metagenome, Molecular Sequence Data, Viral Proteins},
	issn = {2041-1723},
	doi = {10.1038/ncomms5498},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/25058116},
	author = {Dutilh, Bas E and Cassman, Noriko and McNair, Katelyn and Sanchez, Savannah E and Silva, Genivaldo G Z and Boling, Lance and Barr, Jeremy J and Speth, Daan R and Seguritan, Victor and Aziz, Ramy K and Felts, Ben and Dinsdale, Elizabeth A and Mokili, John L and Edwards, Robert A}
}
