@article {309771,
	title = {"Bar-coding" primate chromosomes: molecular cytogenetic screening for the ancestral hominoid karyotype.},
	journal = {Hum Genet},
	volume = {109},
	year = {2001},
	month = {2001 Jul},
	pages = {85-94},
	abstract = {<p>Two recently introduced multicolor FISH approaches, cross-species color banding (also termed Rx-FISH) and multiplex FISH using painting probes derived from somatic cell hybrids retaining fragments of human chromosomes, were applied in a comparative molecular cytogenetic study of higher primates. We analyzed these "chromosome bar code" patterns to obtain an overview of chromosomal rearrangements that occurred during higher primate evolution. The objective was to reconstruct the ancestral genome organization of hominoids using the macaque as outgroup species. Approximately 160 individual and discernible molecular cytogenetic markers were assigned in these species. Resulting comparative maps allowed us to identify numerous intra-chromosomal rearrangements, to discriminate them from previous contradicting chromosome banding interpretations and to propose an ancestral karyotype for hominoids. From 25 different chromosome forms in an ancestral karyotype for all hominoids of 2N=48 we propose 21. Probes for chromosomes 2p, 4, 9 and Y were not informative in the present experiments. The orangutan karyotype was very similar to the proposed ancestral organization and conserved 19 of the 21 ancestral forms; thus most chromosomes were already present in early hominoid evolution, while African apes and human show various derived changes.</p>
},
	keywords = {Animals, Cell Line, Chromosome Painting, Chromosomes, Chromosomes, Human, Cytogenetics, Gorilla gorilla, Hominidae, Humans, Hylobates, In Situ Hybridization, Fluorescence, Karyotyping, Macaca nemestrina, Pan troglodytes, Pongo pygmaeus, Primates, Species Specificity},
	issn = {0340-6717},
	author = {M{\"u}ller, S and Wienberg, J}
}
