@article {314773,
	title = {Single-cell methylomes identify neuronal subtypes and regulatory elements in mammalian cortex.},
	journal = {Science},
	volume = {357},
	number = {6351},
	year = {2017},
	month = {Aug 11},
	pages = {600-604},
	abstract = {<p>The mammalian brain contains diverse neuronal types, yet we lack single-cell epigenomic assays that are able to identify and characterize them. DNA methylation is a stable epigenetic mark that distinguishes cell types and marks regulatory elements. We generated \&gt;6000 methylomes from single neuronal nuclei and used them to identify 16 mouse and 21 human neuronal subpopulations in the frontal cortex. CG and non-CG methylation exhibited cell type-specific distributions, and we identified regulatory elements with differential methylation across neuron types. Methylation signatures identified a layer 6 excitatory neuron subtype and a unique human parvalbumin-expressing inhibitory neuron subtype. We observed stronger cross-species conservation of regulatory elements in inhibitory neurons than in excitatory neurons. Single-nucleus methylomes expand the atlas of brain cell types and identify regulatory elements that drive conserved brain cell diversity.</p>
},
	isbn = {0036-8075},
	url = {https://www.ncbi.nlm.nih.gov/pubmed/28798132},
	author = {Luo, C and Keown, CL and Kurihara, L and Zhou, J and He, Y and Li, J and Castanon, R and Lucero, J and Nery, JR and Sandoval, JP and Bui, B and Sejnowski, TJ and Harkins, TT and Mukamel, EA and Behrens, MM and Ecker, JR}
}
