@article {327115,
	title = {Altered Neuronal Support and Inflammatory Response in Bipolar Disorder Patient-Derived Astrocytes.},
	journal = {Stem Cell Reports},
	volume = {16},
	year = {2021},
	month = {2021 Apr 13},
	pages = {825-835},
	abstract = {<p>Bipolar disorder (BD) is characterized by cyclical mood shifts. Studies indicate that BD patients have a peripheral pro-inflammatory state and alterations in glial populations in the brain. We utilized an in\&nbsp;vitro model to study inflammation-related phenotypes of astrocytes derived from induced pluripotent stem cells (iPSCs) generated from BD patients and healthy controls. BD astrocytes showed changes in transcriptome and induced a reduction in neuronal activity when co-cultured with neurons. IL-1β-stimulated BD astrocytes displayed a unique inflammatory gene expression signature and increased secretion of IL-6. Conditioned medium from stimulated BD astrocytes reduced neuronal activity, and this effect was partially blocked by IL-6 inactivating antibody. Our results suggest that BD astrocytes are functionally less supportive of neuronal excitability and this effect is partially mediated by IL-6. We confirmed higher IL-6 in blood in a distinct cohort of BD patients, highlighting the potential role of astrocyte-mediated inflammatory signaling in BD neuropathology.</p>
},
	keywords = {Astrocytes, Bipolar Disorder, Coculture Techniques, Humans, Induced Pluripotent Stem Cells, Inflammation, Interleukin-1beta, Interleukin-6, Neuroglia, Neurons},
	issn = {2213-6711},
	doi = {10.1016/j.stemcr.2021.02.004},
	author = {Vadodaria, Krishna C and Mendes, Ana P D and Mei, Arianna and Racha, Vipula and Erikson, Galina and Shokhirev, Maxim N and Oefner, Ruth and Heard, Kelly J and McCarthy, Michael J and Eyler, Lisa and Kelsoe, John R and Santos, Renata and Marchetto, Maria C and Gage, Fred H}
}
