@article {328965,
	title = {Evolutionary origins and interactomes of human, young microproteins and small peptides translated from short open reading frames},
	year = {2023},
	abstract = {<p>All species continuously evolve short open reading frames (sORFs) that can be templated for protein synthesis and may provide raw materials for evolutionary adaptation. We analyzed the evolutionary origins of 7,264 recently cataloged human sORFs and found that most were evolutionarily young and had emerged de novo. We additionally identified 221 previously missed sORFs potentially translated into peptides of up to 15 amino acids{\textemdash}all of which are smaller than the smallest human microprotein annotated to date. To investigate the bioactivity of sORF-encoded small peptides and young microproteins, we subjected 266 candidates to a mass-spectrometry-based interactome screen with motif resolution. Based on these interactomes and additional cellular assays, we can associate several candidates with mRNA splicing, translational regulation, and endocytosis. Our work provides insights into the evolutionary origins and interaction potential of young and small proteins, thereby helping to elucidate this underexplored territory of the human proteome.</p>
},
	url = {https://www.sciencedirect.com/science/article/pii/S1097276523000758},
	author = {Clara-L. Sandmann and Jana F. Schulz and Jorge Ruiz-Orera and Marieluise Kirchner and Matthias Ziehm and Eleonora Adami and Maike Marczenke and Annabel Christ and Nina Liebe and Johannes Greiner and Aaron Schoenenberger and Michael B. Muecke and Ning Liang and Robert L. Moritz and Zhi Sun and Eric W. Deutsch and Michael Gotthardt and Jonathan M. Mudge and John R. Prensner and Thomas E. Willnow and Philipp Mertins and Sebastiaan van Heesch and Norbert Hubner}
}
