@article {329055,
	title = {Utilizing nanopore sequencing technology for the rapid and comprehensive characterization of eleven HLA loci; addressing the need for deceased donor expedited HLA typing.},
	journal = {Hum Immunol},
	volume = {81},
	year = {2020},
	month = {2020 Aug},
	pages = {413-422},
	abstract = {<p>The comprehensive characterization of human leukocyte antigen (HLA) genomic sequences remains a challenging problem. Despite the significant advantages of next-generation sequencing (NGS) in the field of Immunogenetics, there has yet to be a single solution for unambiguous, accurate, simple, cost-effective, and timely genotyping necessary for all clinical applications. This report demonstrates the benefits of nanopore sequencing introduced by Oxford Nanopore Technologies (ONT) for HLA genotyping. Samples (n\&nbsp;=\&nbsp;120) previously characterized at high-resolution three-field (HR-3F) for 11 loci were assessed using ONT sequencing paired to a single-plex PCR protocol (Holotype) and to two multiplex protocols OmniType (Omixon) and NGSgo{\textregistered}-MX6-1 (GenDx). The results demonstrate the potential of nanopore sequencing for delivering accurate HR-3F typing with a simple, rapid, and cost-effective protocol. The protocol is applicable to time-sensitive applications, such as deceased donor typings, enabling better assessments of compatibility and epitope analysis. The technology also allows significantly shorter turnaround time for multiple samples at a lower cost. Overall, the nanopore technology appears to offer a significant advancement over current next-generation sequencing platforms as a single solution for all HLA genotyping needs.</p>
},
	keywords = {Alleles, Genomics, Genotyping Techniques, High-Throughput Nucleotide Sequencing, Histocompatibility Testing, HLA Antigens, Humans, Nanopore Sequencing, Sequence Analysis, DNA, Tissue Donors},
	issn = {1879-1166},
	doi = {10.1016/j.humimm.2020.06.004},
	author = {Mosbruger, Timothy L and Dinou, Amalia and Duke, Jamie L and Ferriola, Deborah and Mehler, Hilary and Pagkrati, Ioanna and Damianos, Georgios and Mbunwe, Eric and Sarmady, Mahdi and Lyratzakis, Ioannis and Tishkoff, Sarah A and Dinh, Anh and Monos, Dimitri S}
}
