@article {330521,
	title = {Whole-brain annotation and multi-connectome cell typing of Drosophila},
	volume = {634},
	year = {2024},
	month = {2024/10/01},
	pages = {139 - 152},
	abstract = {<p>The fruit fly Drosophila melanogaster has emerged as a key model organism in neuroscience, in large part due to the concentration of collaboratively generated molecular, genetic and digital resources available for it. Here we complement the approximately 140,000 neuron FlyWire whole-brain connectome1 with a systematic and hierarchical annotation of neuronal classes, cell types and developmental units (hemilineages). Of 8,453 annotated cell types, 3,643 were previously proposed in the partial hemibrain connectome2, and 4,581 are new types, mostly from brain regions outside the hemibrain subvolume. Although nearly all hemibrain neurons could be matched morphologically in FlyWire, about one-third of cell types proposed for the hemibrain could not be reliably reidentified. We therefore propose a new definition of cell type as groups of cells that are each quantitatively more similar to cells in a different brain than to any other cell in the same brain, and we validate this definition through joint analysis of FlyWire and hemibrain connectomes. Further analysis defined simple heuristics for the reliability of connections between brains, revealed broad stereotypy and occasional variability in neuron count and connectivity, and provided evidence for functional homeostasis in the mushroom body through adjustments of the absolute amount of excitatory input while maintaining the excitation/inhibition ratio. Our work defines a consensus cell type atlas for the fly brain and provides both an intellectual framework and open-source toolchain for brain-scale comparative connectomics.</p>
},
	isbn = {1476-4687},
	doi = {https://doi.org/10.1038/s41586-024-07686-5},
	url = {https://www.nature.com/articles/s41586-024-07686-5},
	author = {Schlegel, Philipp and Yin, Yijie and Bates, Alexander S. and Dorkenwald, Sven and Eichler, Katharina and Brooks, Paul and Han, Daniel S. and Gkantia, Marina and dos Santos, Marcia and Munnelly, Eva J. and Badalamente, Griffin and Serratosa Capdevila, Laia and Sane, Varun A. and Fragniere, Alexandra M. C. and Kiassat, Ladann and Pleijzier, Markus W. and St{\"u}rner, Tomke and Tamimi, Imaan F. M. and Dunne, Christopher R. and Salgarella, Irene and Javier, Alexandre and Fang, Siqi and Perlman, Eric and Kazimiers, Tom and Jagannathan, Sridhar R. and Matsliah, Arie and Sterling, Amy R. and Yu, Szi-chieh and McKellar, Claire E. and Kruk, Krzysztof and Bland, Doug and Lenizo, Zairene and Burke, Austin T. and Willie, Kyle Patrick and Bates, Alexander S. and Serafetinidis, Nikitas and Hadjerol, Nashra and Willie, Ryan and Silverman, Ben and Ocho, John Anthony and Ba{\~n}ez, Joshua and Candilada, Rey Adrian and Gager, Jay and Kristiansen, Anne and Panes, Nelsie and Yadav, Arti and Tancontian, Remer and Serona, Shirleyjoy and Dolorosa, Jet Ivan and Vinson, Kendrick Joules and Garner, Dustin and Salem, Regine and Dagohoy, Ariel and Skelton, Jaime and Lopez, Mendell and Stocks, Thomas and Pandey, Anjali and Akiatan, Darrel Jay and Hebditch, James and David, Celia and Sapkal, Dharini and Monungolh, Shaina Mae and Sane, Varun and Pielago, Mark Lloyd and Albero, Miguel and Laude, Jacquilyn and dos Santos, Marcia and Deutsch, David and Vohra, Zeba and Wang, Kaiyu and Gogo, Allien Mae and Kind, Emil and Mandahay, Alvin Josh and Martinez, Chereb and Asis, John David and Nair, Chitra and Patel, Dhwani and Manaytay, Marchan and Lim, Clyde Angelo and Ampo, Philip Lenard and Pantujan, Michelle Darapan and Bautista, Daril and Rana, Rashmita and Seguido, Jansen and Parmar, Bhargavi and Saguimpa, John Clyde and Moore, Merlin and Pleijzier, Markus W. and Larson, Mark and Hsu, Joseph and Joshi, Itisha and Kakadiya, Dhara and Braun, Amalia and Pilapil, Cathy and Parmar, Kaushik and Vanderbeck, Quinn and Dunne, Christopher and Munnelly, Eva and Kang, Chan Hyuk and L{\"o}rsch, Lena and Lee, Jinmook and Kmecova, Lucia and Sancer, Gizem and Baker, Christa and Joroff, Jenna and Calle, Steven and Patel, Yashvi and Sato, Olivia and Salocot, Janice and Salman, Farzaan and Molina-Obando, Sebastian and Bui, Mai and Lichtenberger, Matthew and Tamboboy, Edmark and Molloy, Katie and Santana-Cruz, Alexis E. and Hernandez, Anthony and Yu, Seongbong and Sorek, Marissa and Diwan, Arzoo and Patel, Monika and Aiken, Travis R. and Morejohn, Sarah and Koskela, Sanna and Yang, Tansy and Lehmann, Daniel and Chojetzki, Jonas and Sisodiya, Sangeeta and Koolman, Selden and Shiu, Philip K. and Cho, Sky and Bast, Annika and Reicher, Brian and Blanquart, Marlon and Houghton, Lucy and Choi, Hyungjun and Ioannidou, Maria and Collie, Matt and Eckhardt, Joanna and Gorko, Benjamin and Guo, Li and Zheng, Zhihao and Poh, Alisa and Lin, Marina and Taisz, Istv{\'a}n and Murfin, Wes and D{\'\i}ez, {\'A}lvaro Sanz and Reinhard, Nils and Gibb, Peter and Patel, Nidhi and Kumar, Sandeep and Yun, Minsik and Wang, Megan and Jones, Devon and Encarnacion-Rivera, Lucas and Oswald, Annalena and Jadia, Akanksha and Erginkaya, Mert and Drummond, Nik and Walter, Leonie and Tastekin, Ibrahim and Zhong, Xin and Mabuchi, Yuta and Figueroa Santiago, Fernando J. and Verma, Urja and Byrne, Nick and Kunze, Edda and Crahan, Thomas and Margossian, Ryan and Kim, Haein and Georgiev, Iliyan and Szorenyi, Fabianna and Adachi, Atsuko and Bargeron, Benjamin and St{\"u}rner, Tomke and Demarest, Damian and G{\"u}r, Burak and Becker, Andrea N. and Turnbull, Robert and Morren, Ashley and Sandoval, Andrea and Moreno-Sanchez, Anthony and Pacheco, Diego A. and Samara, Eleni and Croke, Haley and Thomson, Alexander and Laughland, Connor and Dutta, Suchetana B. and de Ant{\'o}n, Paula Guiomar Alarc{\'o}n and Huang, Binglin and Pujols, Patricia and Haber, Isabel and Gonz{\'a}lez-Segarra, Amanda and Lin, Albert and Choe, Daniel T. and Lukyanova, Veronika and Mancini, Nino and Liu, Zequan and Okubo, Tatsuo and Flynn, Miriam A. and Vitelli, Gianna and Laturney, Meghan and Li, Feng and Cao, Shuo and Manyari-Diaz, Carolina and Yim, Hyunsoo and Duc Le, Anh and Maier, Kate and Yu, Seungyun and Nam, Yeonju and B{\k a}ba, Daniel and Abusaif, Amanda and Francis, Audrey and Gayk, Jesse and Huntress, Sommer S. and Barajas, Raquel and Kim, Mindy and Cui, Xinyue and Sterling, Amy R. and Sterne, Gabriella R. and Li, Anna and Park, Keehyun and Dempsey, Georgia and Mathew, Alan and Kim, Jinseong and Kim, Taewan and Wu, Guan-ting and Dhawan, Serene and Brotas, Margarida and Zhang, Cheng-hao and Bailey, Shanice and Del Toro, Alexander and Lee, Kisuk and Macrina, Thomas and Schneider-Mizell, Casey and Popovych, Sergiy and Ogedengbe, Oluwaseun and Yang, Runzhe and Halageri, Akhilesh and Silversmith, Will and Gerhard, Stephan and Champion, Andrew and Eckstein, Nils and Ih, Dodam and Kemnitz, Nico and Castro, Manuel and Jia, Zhen and Wu, Jingpeng and Mitchell, Eric and Nehoran, Barak and Mu, Shang and Bae, J. Alexander and Lu, Ran and Morey, Ryan and Kuehner, Kai and Brittain, Derrick and Jordan, Chris S. and Anderson, David J. and Behnia, Rudy and Bidaye, Salil S. and Borst, Alexander and Chiappe, Eugenia and Collman, Forrest and Colodner, Kenneth J. and Dacks, Andrew and Dickson, Barry and Funke, Jan and Garcia, Denise and Hampel, Stefanie and Hartenstein, Volker and Hassan, Bassem and Helfrich-Forster, Charlotte and Huetteroth, Wolf and Kim, Jinseop and Kim, Sung Soo and Kim, Young-Joon and Kwon, Jae Young and Lee, Wei-Chung and Linneweber, Gerit A. and Maimon, Gaby and Mann, Richard and Noselli, St{\'e}phane and Pankratz, Michael and Prieto-Godino, Lucia and Read, Jenny and Reiser, Michael and von Reyn, Katie and Ribeiro, Carlos and Scott, Kristin and Seeds, Andrew M. and Selcho, Mareike and Silies, Marion and Simpson, Julie and Waddell, Scott and Wernet, Mathias F. and Wilson, Rachel I. and Wolf, Fred W. and Yao, Zepeng and Yapici, Nilay and FlyWire Consortium}
}
