%0 Journal Article %J J Chem Neuroanat %D 1997 %T Colocalization of parvalbumin and calbindin D-28k in neurons including chandelier cells of the human temporal neocortex. %A del Río, M R %A DeFelipe, J %K Adolescent %K Adult %K Calbindins %K Fluorescent Antibody Technique, Direct %K Humans %K Immunohistochemistry %K Neurons %K Parvalbumins %K Presynaptic Terminals %K S100 Calcium Binding Protein G %K Temporal Lobe %X

Chandelier cells are cortical GABAergic interneurons with a unique synaptic specificity enabling them to exert a strong inhibitory influence on pyramidal cells. By using immunocytochemistry for the calcium-binding protein calbindin D-28k in the human temporal neocortex, we have found numerous immunoreactive processes that were identified as chandelier cell axon terminals. This was a striking find since in previous immunocytochemical studies of the primate neocortex, chandelier cell axon terminals had been shown to be immunoreactive for another calcium-binding protein, parvalbumin, and colocalization studies indicate that parvalbumin and calbindin are present in almost completely separate neuronal populations. Here, we present double-label immunofluorescence experiments showing that parvalbumin and calbindin immunoreactivities are colocalized in certain neurons that include a subpopulation of chandelier cells whose cell bodies are located mainly in layers V and VI of the human temporal neocortex. The results suggest a selective laminar distribution of neurochemical subtypes of chandelier cells which is a peculiar feature of the organization of the human neocortex.

%B J Chem Neuroanat %V 12 %P 165-73 %8 1997 Mar %G eng %N 3 %1

http://www.ncbi.nlm.nih.gov/pubmed/9141648?dopt=Abstract