<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Alisch, R. S.</style></author><author><style face="normal" font="default" size="100%">Garcia-Perez, J. L.</style></author><author><style face="normal" font="default" size="100%">Muotri, A. R.</style></author><author><style face="normal" font="default" size="100%">Gage, F. H.</style></author><author><style face="normal" font="default" size="100%">Moran, J. V.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Unconventional translation of mammalian LINE-1 retrotransposons</style></title><secondary-title><style face="normal" font="default" size="100%">Genes Dev</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Genes &amp; development</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Base Sequence</style></keyword><keyword><style  face="normal" font="default" size="100%">Codon</style></keyword><keyword><style  face="normal" font="default" size="100%">Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">HeLa Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Initiator/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Long Interspersed Nucleotide Elements/*genetics/physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Models</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">Open Reading Frames/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Peptide Chain Init</style></keyword><keyword><style  face="normal" font="default" size="100%">Terminator/genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan 15</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/16418485</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">2</style></number><edition><style face="normal" font="default" size="100%">2006/01/19</style></edition><volume><style face="normal" font="default" size="100%">20</style></volume><pages><style face="normal" font="default" size="100%">210-24</style></pages><isbn><style face="normal" font="default" size="100%">0890-9369 (Print)0890-93</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Long Interspersed Element-1 (LINE-1 or L1) retrotransposons encode proteins required for their mobility (ORF1p and ORF2p), yet little is known about how L1 mRNA is translated. Here, we show that ORF2 translation generally initiates from the first in-frame methionine codon of ORF2, and that both ORF1 and the inter-ORF spacer are dispensable for ORF2 translation. Remarkably, changing the ORF2 AUG codon to any other coding triplet is compatible with retrotransposition. However, introducing a premature termination codon in ORF1 or a thermostable hairpin in the inter-ORF spacer reduces ORF2p translation or L1 retrotransposition to approximately 5% of wild-type levels. Similar data obtained from &amp;quot;natural&amp;quot; and codon optimized &amp;quot;synthetic&amp;quot; mouse L1s lead us to propose that ORF2 is translated by an unconventional termination/reinitiation mechanism.&lt;/p&gt;</style></abstract><work-type><style face="normal" font="default" size="100%">Research Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov&#039;t</style></work-type><accession-num><style face="normal" font="default" size="100%">16418485</style></accession-num><notes><style face="normal" font="default" size="100%">&lt;p&gt;Genes Dev. 2006 Jan 15;20(2):210-24&lt;/p&gt;</style></notes><custom2><style face="normal" font="default" size="100%">1356112</style></custom2><auth-address><style face="normal" font="default" size="100%">Department of Human Genetics and Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109-0618, USA.</style></auth-address></record></records></xml>