<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Coufal, N. G.</style></author><author><style face="normal" font="default" size="100%">Garcia-Perez, J. L.</style></author><author><style face="normal" font="default" size="100%">Peng, G. E.</style></author><author><style face="normal" font="default" size="100%">Yeo, G. W.</style></author><author><style face="normal" font="default" size="100%">Mu, Y.</style></author><author><style face="normal" font="default" size="100%">Lovci, M. T.</style></author><author><style face="normal" font="default" size="100%">Morell, M.</style></author><author><style face="normal" font="default" size="100%">O&#039;Shea, K. S.</style></author><author><style face="normal" font="default" size="100%">Moran, J. V.</style></author><author><style face="normal" font="default" size="100%">Gage, F. H.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">L1 retrotransposition in human neural progenitor cells</style></title><secondary-title><style face="normal" font="default" size="100%">Nature</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Nature</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">&#039;</style></keyword><keyword><style  face="normal" font="default" size="100%">5</style></keyword><keyword><style  face="normal" font="default" size="100%">Brain/cytology</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Line</style></keyword><keyword><style  face="normal" font="default" size="100%">Chromatin Immunoprecipitation</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA Methylation</style></keyword><keyword><style  face="normal" font="default" size="100%">Embryonic Stem Cells/*cytology/*metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Fetus/cytology</style></keyword><keyword><style  face="normal" font="default" size="100%">Gene Dosage</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Neurons/*cytology/*metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Ret</style></keyword><keyword><style  face="normal" font="default" size="100%">Untranslated Regions/genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Aug 27</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/19657334</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">7259</style></number><edition><style face="normal" font="default" size="100%">2009/08/07</style></edition><volume><style face="normal" font="default" size="100%">460</style></volume><pages><style face="normal" font="default" size="100%">1127-31</style></pages><isbn><style face="normal" font="default" size="100%">1476-4687 (Electronic)00</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;Long interspersed element 1 (LINE-1 or L1) retrotransposons have markedly affected the human genome. L1s must retrotranspose in the germ line or during early development to ensure their evolutionary success, yet the extent to which this process affects somatic cells is poorly understood. We previously demonstrated that engineered human L1s can retrotranspose in adult rat hippocampus progenitor cells in vitro and in the mouse brain in vivo. Here we demonstrate that neural progenitor cells isolated from human fetal brain and derived from human embryonic stem cells support the retrotransposition of engineered human L1s in vitro. Furthermore, we developed a quantitative multiplex polymerase chain reaction that detected an increase in the copy number of endogenous L1s in the hippocampus, and in several regions of adult human brains, when compared to the copy number of endogenous L1s in heart or liver genomic DNAs from the same donor. These data suggest that de novo L1 retrotransposition events may occur in the human brain and, in principle, have the potential to contribute to individual somatic mosaicism.&lt;/p&gt;</style></abstract><work-type><style face="normal" font="default" size="100%">Research Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov&#039;t</style></work-type><notes><style face="normal" font="default" size="100%">&lt;p&gt;&lt;span role=&quot;menubar&quot;&gt;Nature.&lt;/span&gt;&amp;nbsp;2009 Aug 27;460(7259):1127-31. doi: 10.1038/nature08248. Epub 2009 Aug 5.&lt;/p&gt;</style></notes><custom2><style face="normal" font="default" size="100%">2909034</style></custom2><auth-address><style face="normal" font="default" size="100%">Laboratory of Genetics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA.</style></auth-address></record></records></xml>