<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Zhang, Ye</style></author><author><style face="normal" font="default" size="100%">Sloan, Steven A</style></author><author><style face="normal" font="default" size="100%">Clarke, Laura E</style></author><author><style face="normal" font="default" size="100%">Caneda, Christine</style></author><author><style face="normal" font="default" size="100%">Plaza, Colton A</style></author><author><style face="normal" font="default" size="100%">Blumenthal, Paul D</style></author><author><style face="normal" font="default" size="100%">Vogel, Hannes</style></author><author><style face="normal" font="default" size="100%">Steinberg, Gary K</style></author><author><style face="normal" font="default" size="100%">Edwards, Michael S B</style></author><author><style face="normal" font="default" size="100%">Li, Gordon</style></author><author><style face="normal" font="default" size="100%">Duncan, John A</style></author><author><style face="normal" font="default" size="100%">Cheshier, Samuel H</style></author><author><style face="normal" font="default" size="100%">Shuer, Lawrence M</style></author><author><style face="normal" font="default" size="100%">Chang, Edward F</style></author><author><style face="normal" font="default" size="100%">Grant, Gerald A</style></author><author><style face="normal" font="default" size="100%">Gephart, Melanie G Hayden</style></author><author><style face="normal" font="default" size="100%">Barres, Ben A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Purification and Characterization of Progenitor and Mature Human Astrocytes Reveals Transcriptional and Functional Differences with Mouse.</style></title><secondary-title><style face="normal" font="default" size="100%">Neuron</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Neuron</style></alt-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Astrocytes</style></keyword><keyword><style  face="normal" font="default" size="100%">Brain</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Culture Techniques</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Differentiation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Separation</style></keyword><keyword><style  face="normal" font="default" size="100%">Cell Survival</style></keyword><keyword><style  face="normal" font="default" size="100%">Cells, Cultured</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Microglia</style></keyword><keyword><style  face="normal" font="default" size="100%">Neurons</style></keyword><keyword><style  face="normal" font="default" size="100%">Oligodendroglia</style></keyword><keyword><style  face="normal" font="default" size="100%">Stem Cells</style></keyword><keyword><style  face="normal" font="default" size="100%">Transcriptome</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2016</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2016 Jan 6</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/26687838</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">89</style></volume><pages><style face="normal" font="default" size="100%">37-53</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">&lt;p&gt;The functional and molecular similarities and distinctions between human and murine astrocytes are poorly understood. Here, we report the development of an immunopanning method to acutely purify astrocytes from fetal, juvenile, and adult human brains and to maintain these cells in serum-free cultures. We found that human astrocytes have abilities similar to those of murine astrocytes in promoting neuronal survival, inducing functional synapse formation, and engulfing synaptosomes. In contrast to existing observations in mice, we found that mature human astrocytes respond robustly to glutamate. Next, we performed RNA sequencing of healthy human astrocytes along with astrocytes from epileptic and tumor foci and compared these to human neurons, oligodendrocytes, microglia, and endothelial cells (available at http://www.brainrnaseq.org). With these profiles, we identified novel human-specific astrocyte genes and discovered a transcriptome-wide transformation between astrocyte precursor cells and mature post-mitotic astrocytes. These data represent some of the first cell-type-specific molecular profiles of the healthy and diseased human brain.&lt;/p&gt;</style></abstract><issue><style face="normal" font="default" size="100%">1</style></issue><notes><style face="normal" font="default" size="100%">http://www.sciencedirect.com/science/article/pii/S0896627315010193</style></notes><custom1><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/26687838?dopt=Abstract</style></custom1></record></records></xml>