Deletion polymorphism of SIGLEC14 and its functional implications.

Bibliographic Collection: 
MOCA Reference, APE
Publication Type: Journal Article
Authors: Yamanaka, Masahiro; Kato, Yukinari; Angata, Takashi; Narimatsu, Hisashi
Year of Publication: 2009
Journal: Glycobiology
Volume: 19
Issue: 8
Pagination: 841-6
Date Published: 2009 Aug
Publication Language: eng
ISSN: 1460-2423
Keywords: Antigens, CD, Antigens, Differentiation, Myelomonocytic, B-Lymphocytes, Cell Differentiation, Cell Line, Gene Fusion, Granulocytes, Humans, Lectins, Lipopolysaccharides, Macrophages, Monocytes, Polymorphism, Genetic, Receptors, Cell Surface, Tumor Necrosis Factor-alpha

Human Siglec-14, a member of the Siglec family of sialic acid-binding lectins, shows extensive sequence similarity to human Siglec-5. To analyze respective expression patterns of Siglec-14 and Siglec-5, we developed specific antibodies against each of them. We found that the former was expressed on granulocytes and monocytes, while the latter was on granulocytes and B-cells. Surprisingly, some individuals lacked the expression of Siglec-14, while they all expressed Siglec-5. We found that a fusion between SIGLEC14 and SIGLEC5 genes, resulting in the functional deletion of SIGLEC14, underlies this phenotype. The presence of the "SIGLEC14 null" allele in all human populations we tested implies an ancient origin, while its allelic frequency is higher in Asians compared with Africans and Europeans. The forced expression of Siglec-14 in a monocytic cell line-enhanced TNF-alpha secretion elicited by lipopolysaccharide. These results imply that Siglec-14 may play some role in bacterial infection.

DOI: 10.1093/glycob/cwp052
Alternate Journal: Glycobiology