LINE-1 retrotransposition in human embryonic stem cells

Bibliographic Collection:

CARTA-Inspired Publication

Publication Type: Journal Article

Authors: Garcia-Perez, J. L.; Marchetto, M. C.; Muotri, A. R.; Coufal, N. G.; Gage, F. H.; O'Shea, K. S.; Moran, J. V.

Year of Publication: 2007

Journal: Hum Mol Genet

Volume: 16

Edition: 2007/05/01

Number: 13

Pagination: 1569-77

Date Published: Jul 1

Type of Article: Research Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't

Publication Language: eng

ISBN Number: 0964-6906 (Print)0964-69

Accession Number: 17468180

Keywords: Cell Differentiation, Cell Line, DNA/metabolism, Embryonic Stem Cells/*metabolism, Evolution, Genetic, Genome, Humans, Long Interspersed Nucleotide Elements/*genetics, Microscopy, Models, Molecular, P, Phase-Contrast, Plasmids/metabolism, Tumor

Abstract:

LINE-1 elements comprise approximately 17% of human DNA and their mobility continues to impact genome evolution. However, little is known about the types of non-transformed cells that can support LINE-1 retrotransposition. Here, we show that human embryonic stem cells express endogenous LINE-1 elements and can accommodate LINE-1 retrotransposition in vitro. The resultant retrotransposition events can occur into genes and can result in the concomitant deletion of genomic DNA at the target site. Thus, these data suggest that LINE-1 retrotransposition events may occur during early stages of human development.

Notes:

Hum Mol Genet. 2007 Jul 1;16(13):1569-77. Epub 2007 Apr 27

URL: http://www.ncbi.nlm.nih.gov/pubmed/17468180

Alternate Journal: Human molecular genetics

Author Address:

Departments of Human Genetics and Internal Medicine, University of Michigan Medical School, 1241 E. Catherine Street, Ann Arbor, MI 48109, USA.

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