Publication Type: Journal Article
Authors: Schlegel, Philipp; Yin, Yijie; Bates, Alexander S.; Dorkenwald, Sven; Eichler, Katharina; Brooks, Paul; Han, Daniel S.; Gkantia, Marina; dos Santos, Marcia; Munnelly, Eva J.; Badalamente, Griffin; Serratosa Capdevila, Laia; Sane, Varun A.; Fragniere, Alexandra M. C.; Kiassat, Ladann; Pleijzier, Markus W.; Stürner, Tomke; Tamimi, Imaan F. M.; Dunne, Christopher R.; Salgarella, Irene; Javier, Alexandre; Fang, Siqi; Perlman, Eric; Kazimiers, Tom; Jagannathan, Sridhar R.; Matsliah, Arie; Sterling, Amy R.; Yu, Szi-chieh; McKellar, Claire E.; Kruk, Krzysztof; Bland, Doug; Lenizo, Zairene; Burke, Austin T.; Willie, Kyle Patrick; Bates, Alexander S.; Serafetinidis, Nikitas; Hadjerol, Nashra; Willie, Ryan; Silverman, Ben; Ocho, John Anthony; Bañez, Joshua; Candilada, Rey Adrian; Gager, Jay; Kristiansen, Anne; Panes, Nelsie; Yadav, Arti; Tancontian, Remer; Serona, Shirleyjoy; Dolorosa, Jet Ivan; Vinson, Kendrick Joules; Garner, Dustin; Salem, Regine; Dagohoy, Ariel; Skelton, Jaime; Lopez, Mendell; Stocks, Thomas; Pandey, Anjali; Akiatan, Darrel Jay; Hebditch, James; David, Celia; Sapkal, Dharini; Monungolh, Shaina Mae; Sane, Varun; Pielago, Mark Lloyd; Albero, Miguel; Laude, Jacquilyn; dos Santos, Marcia; Deutsch, David; Vohra, Zeba; Wang, Kaiyu; Gogo, Allien Mae; Kind, Emil; Mandahay, Alvin Josh; Martinez, Chereb; Asis, John David; Nair, Chitra; Patel, Dhwani; Manaytay, Marchan; Lim, Clyde Angelo; Ampo, Philip Lenard; Pantujan, Michelle Darapan; Bautista, Daril; Rana, Rashmita; Seguido, Jansen; Parmar, Bhargavi; Saguimpa, John Clyde; Moore, Merlin; Pleijzier, Markus W.; Larson, Mark; Hsu, Joseph; Joshi, Itisha; Kakadiya, Dhara; Braun, Amalia; Pilapil, Cathy; Parmar, Kaushik; Vanderbeck, Quinn; Dunne, Christopher; Munnelly, Eva; Kang, Chan Hyuk; Lörsch, Lena; Lee, Jinmook; Kmecova, Lucia; Sancer, Gizem; Baker, Christa; Joroff, Jenna; Calle, Steven; Patel, Yashvi; Sato, Olivia; Salocot, Janice; Salman, Farzaan; Molina-Obando, Sebastian; Bui, Mai; Lichtenberger, Matthew; Tamboboy, Edmark; Molloy, Katie; Santana-Cruz, Alexis E.; Hernandez, Anthony; Yu, Seongbong; Sorek, Marissa; Diwan, Arzoo; Patel, Monika; Aiken, Travis R.; Morejohn, Sarah; Koskela, Sanna; Yang, Tansy; Lehmann, Daniel; Chojetzki, Jonas; Sisodiya, Sangeeta; Koolman, Selden; Shiu, Philip K.; Cho, Sky; Bast, Annika; Reicher, Brian; Blanquart, Marlon; Houghton, Lucy; Choi, Hyungjun; Ioannidou, Maria; Collie, Matt; Eckhardt, Joanna; Gorko, Benjamin; Guo, Li; Zheng, Zhihao; Poh, Alisa; Lin, Marina; Taisz, István; Murfin, Wes; Díez, Álvaro Sanz; Reinhard, Nils; Gibb, Peter; Patel, Nidhi; Kumar, Sandeep; Yun, Minsik; Wang, Megan; Jones, Devon; Encarnacion-Rivera, Lucas; Oswald, Annalena; Jadia, Akanksha; Erginkaya, Mert; Drummond, Nik; Walter, Leonie; Tastekin, Ibrahim; Zhong, Xin; Mabuchi, Yuta; Figueroa Santiago, Fernando J.; Verma, Urja; Byrne, Nick; Kunze, Edda; Crahan, Thomas; Margossian, Ryan; Kim, Haein; Georgiev, Iliyan; Szorenyi, Fabianna; Adachi, Atsuko; Bargeron, Benjamin; Stürner, Tomke; Demarest, Damian; Gür, Burak; Becker, Andrea N.; Turnbull, Robert; Morren, Ashley; Sandoval, Andrea; Moreno-Sanchez, Anthony; Pacheco, Diego A.; Samara, Eleni; Croke, Haley; Thomson, Alexander; Laughland, Connor; Dutta, Suchetana B.; de Antón, Paula Guiomar Alarcón; Huang, Binglin; Pujols, Patricia; Haber, Isabel; González-Segarra, Amanda; Lin, Albert; Choe, Daniel T.; Lukyanova, Veronika; Mancini, Nino; Liu, Zequan; Okubo, Tatsuo; Flynn, Miriam A.; Vitelli, Gianna; Laturney, Meghan; Li, Feng; Cao, Shuo; Manyari-Diaz, Carolina; Yim, Hyunsoo; Duc Le, Anh; Maier, Kate; Yu, Seungyun; Nam, Yeonju; Bąba, Daniel; Abusaif, Amanda; Francis, Audrey; Gayk, Jesse; Huntress, Sommer S.; Barajas, Raquel; Kim, Mindy; Cui, Xinyue; Sterling, Amy R.; Sterne, Gabriella R.; Li, Anna; Park, Keehyun; Dempsey, Georgia; Mathew, Alan; Kim, Jinseong; Kim, Taewan; Wu, Guan-ting; Dhawan, Serene; Brotas, Margarida; Zhang, Cheng-hao; Bailey, Shanice; Del Toro, Alexander; Lee, Kisuk; Macrina, Thomas; Schneider-Mizell, Casey; Popovych, Sergiy; Ogedengbe, Oluwaseun; Yang, Runzhe; Halageri, Akhilesh; Silversmith, Will; Gerhard, Stephan; Champion, Andrew; Eckstein, Nils; Ih, Dodam; Kemnitz, Nico; Castro, Manuel; Jia, Zhen; Wu, Jingpeng; Mitchell, Eric; Nehoran, Barak; Mu, Shang; Bae, J. Alexander; Lu, Ran; Morey, Ryan; Kuehner, Kai; Brittain, Derrick; Jordan, Chris S.; Anderson, David J.; Behnia, Rudy; Bidaye, Salil S.; Borst, Alexander; Chiappe, Eugenia; Collman, Forrest; Colodner, Kenneth J.; Dacks, Andrew; Dickson, Barry; Funke, Jan; Garcia, Denise; Hampel, Stefanie; Hartenstein, Volker; Hassan, Bassem; Helfrich-Forster, Charlotte; Huetteroth, Wolf; Kim, Jinseop; Kim, Sung Soo; Kim, Young-Joon; Kwon, Jae Young; Lee, Wei-Chung; Linneweber, Gerit A.; Maimon, Gaby; Mann, Richard; Noselli, Stéphane; Pankratz, Michael; Prieto-Godino, Lucia; Read, Jenny; Reiser, Michael; von Reyn, Katie; Ribeiro, Carlos; Scott, Kristin; Seeds, Andrew M.; Selcho, Mareike; Silies, Marion; Simpson, Julie; Waddell, Scott; Wernet, Mathias F.; Wilson, Rachel I.; Wolf, Fred W.; Yao, Zepeng; Yapici, Nilay; FlyWire Consortium
Year of Publication: 2024
Volume: 634
Issue: 8032
Pagination: 139 - 152
Date Published: 2024/10/01
Publication Language: eng
ISBN Number: 1476-4687
Abstract: The fruit fly Drosophila melanogaster has emerged as a key model organism in neuroscience, in large part due to the concentration of collaboratively generated molecular, genetic and digital resources available for it. Here we complement the approximately 140,000 neuron FlyWire whole-brain connectome1 with a systematic and hierarchical annotation of neuronal classes, cell types and developmental units (hemilineages). Of 8,453 annotated cell types, 3,643 were previously proposed in the partial hemibrain connectome2, and 4,581 are new types, mostly from brain regions outside the hemibrain subvolume. Although nearly all hemibrain neurons could be matched morphologically in FlyWire, about one-third of cell types proposed for the hemibrain could not be reliably reidentified. We therefore propose a new definition of cell type as groups of cells that are each quantitatively more similar to cells in a different brain than to any other cell in the same brain, and we validate this definition through joint analysis of FlyWire and hemibrain connectomes. Further analysis defined simple heuristics for the reliability of connections between brains, revealed broad stereotypy and occasional variability in neuron count and connectivity, and provided evidence for functional homeostasis in the mushroom body through adjustments of the absolute amount of excitatory input while maintaining the excitation/inhibition ratio. Our work defines a consensus cell type atlas for the fly brain and provides both an intellectual framework and open-source toolchain for brain-scale comparative connectomics.
DOI: https://doi.org/10.1038/s41586-024-07686-5
Short Title: Nature
