Global epigenomic reconfiguration during mammalian brain development

Bibliographic Collection:

CARTA-Inspired Publication

Publication Type: Journal Article

Authors: Lister, R.; Mukamel, E. A.; Nery, J. R.; Urich, M.; Puddifoot, C. A.; Johnson, N. D.; Lucero, J.; Huang, Y.; Dwork, A. J.; Schultz, M. D.; Yu, M.; Tonti-Filippini, J.; Heyn, H.; Hu, S.; Wu, J. C.; Rao, A.; Esteller, M.; He, C.; Haghighi, F. G.; Sejnowski, T. J.; Behrens, M. M.; Ecker, J. R.

Year of Publication: 2013

Journal: Science

Volume: 341

Edition: 2013/07/06

Number: 6146

Pagination: 1237905

Date Published: Aug 9

Type of Article: Research Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

Publication Language: eng

ISBN Number: 1095-9203 (Electronic)00

Accession Number: 23828890

Keywords: &, *DNA Methylation, *Epigenesis, *Gene Expression Regulation, 5-Methylcytosine/metabolism, Adult, Animals, Base Sequence, Conserved Sequence, Cytosine/*analogs, derivatives/metabolism, Devel, development, Epigenomics, Frontal Lobe/*growth, Genetic

Abstract:

DNA methylation is implicated in mammalian brain development and plasticity underlying learning and memory. We report the genome-wide composition, patterning, cell specificity, and dynamics of DNA methylation at single-base resolution in human and mouse frontal cortex throughout their lifespan. Widespread methylome reconfiguration occurs during fetal to young adult development, coincident with synaptogenesis. During this period, highly conserved non-CG methylation (mCH) accumulates in neurons, but not glia, to become the dominant form of methylation in the human neuronal genome. Moreover, we found an mCH signature that identifies genes escaping X-chromosome inactivation. Last, whole-genome single-base resolution 5-hydroxymethylcytosine (hmC) maps revealed that hmC marks fetal brain cell genomes at putative regulatory regions that are CG-demethylated and activated in the adult brain and that CG demethylation at these hmC-poised loci depends on Tet2 activity.

Notes:

Science. 2013 Aug 9;341(6146):1237905. doi: 10.1126/science.1237905. Epub 2013 Jul 4.

URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3785061/

Custom 2:

3785061

Author Address:

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA. ryan.lister@uwa.edu.au

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